How do I prepare serum and saline tube?

If you do not have our proprietary media tubes handy at the moment, please use the serum and saline protocol from the CSU Hemapathology Lab:

Place 1ml of saline (.9%, LRS, Norm R) into a no-additive, white-top or red-top tube (plain tube with no additives or serum separator).
Add .1 ml of serum from the patient, or another healthy animal of the same species, to the saline in the white-top or red-top tube.Aspirate using suction.Gently squirt contents into the tube containing the saline and serum mixture.
Rinse syringe by drawing up saline and serum mixture and gently squirting it back into the tube. Don’t make bubbles.
Repeat aspiration and rinsing multiple times until solution is no longer transparent. A turbid solution usually indicates adequate cellularity for flow cytometry testing.

Other Questions

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What does flow cytometry tell me about my patient’s specimen?

ImpriMed’s flow cytometry report provides comprehensive information about the specimen’s immunophenotype. B-cell and T-cell immunophenotypes are useful in determining lymphoma/leukemia subtype and prognosis. In addition, our panel of ten antigens can also be used in the diagnosis of T-zonal lymphoma, acute leukemia, and other diseases. Antigens levels reported are: CD21, CD79a, CD3, CD4, CD8, CD5, CD45, CD34, CD14, and MHC class II. For more information, see: https://pubmed.ncbi.nlm.nih.gov/26953614/

How long can the media tubes be outside of that temperature before going bad?

We will send you another batch of media tubes you could use. Please make the supply request on the Vet Portal > Request Supplies page.

For patients who already have flow cytometry, can you just run the drug sensitivity profile?

No, we cannot run a drug sensitivity-only service. Please understand our AI-based drug response prediction models require flow cytometry and PARR parameters generated from our own instrumental setting. Even if the patient already has flow cytometry results from another laboratory, we need to run flow and PARR again anyway.

How long does the media last before expiring? How long is the medium good for?

The media has a 3 month shelf life. You can check the tube and look for its expiration date as well.

Which cancers can I use ImpriMed for? or What type of cancer does ImpriMed predict?

Currently, our services are for canine lymphoma and leukemia. We will be expanding our services to other species and other blood / lymphoid cancers in the near future.